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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
01/11/2018 |
Actualizado : |
07/11/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ZARANTONELLI, L.; SUANES, A.; MENY, P.; BURONI, F.; SALVARREY,X.; BRIANO , C.; ASHFIELD, N.; SILVEIRA, C.S.; DUTRA, F.; EASTON, C.; FRAGA, M.; GIANNITTI, F.; HAMOND, C.; MACÍAS-RIOSECO, M.; MENÉNDEZ, C.; MORTOLA, A.; PICARDEAU, M. |
Afiliación : |
LETICIA ZARANTONELLI, Laboratorio de Microbiología Molecular y Estructural, Institut Pasteur de Montevideo, Uruguay.; Unidad Mixta UMPI, Institut Pasteur de Montevideo; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; ALEJANDRA SUANES, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; PAULINA MENY, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Uruguay.; FLORENCIA BURONI, División Laboratorios Veterinarios "Miguel C. Rubino" Laboratorio Regional Noroeste, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; XIMENA SALVARREY, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino". Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay .; CAROLINA BRIANO, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay .; NATALIA ASHFIELD4, Departamento de Bacteriología y Virologí, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO DUTRA, División Laboratorios Veterinarios "Miguel C. Rubino". Laboratorio Regional Este, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; CRISTINA EASTON, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAMILA HAMOND, Unidad Mixta UMPI, Institut Pasteur de Montevideo ; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLARA MENÉNDEZ, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Uruguay.; ALBERTO MORTOLA, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; MATHIEU PICARDEAU, Institut Pasteur de Montevideo, Uruguay. / Institut Pasteur, France. |
Título : |
Isolation of pathogenic Leptospira strains from naturally infected cattle in Uruguay reveals high serovar diversity, and uncovers a relevant risk for human leptospirosis. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
PLoS Neglected Tropical Diseases, September 2018, vol. 12, Issue 9, Article number e0006694. OPEN ACCESS. |
DOI : |
10.1371/journal.pntd.0006694 |
Idioma : |
Inglés |
Notas : |
Article History: Received: February 8, 2018; Accepted: July 16, 2018; Published: September 13, 2018. |
Contenido : |
Abstract:
Leptospirosis is a neglected zoonosis with worldwide distribution. The causative agents are spirochete bacteria of the Leptospira genus, displaying huge diversity of serovars, the identity of which is critical for effective diagnosis and vaccination purposes. Among many other mammalian species, Leptospira infects cattle, eliciting acute signs in calves, and chronic disease in adult animals often leading to abortions. In South America, and including in Uruguay, beef and dairy export are leading sources of national income. Despite the importance of bovine health, food safety, and bovine-related dissemination of leptospirosis to humans, extremely limited information is available as to the identity of Leptospira species and serovars infecting cattle in Uruguay and the South American subcontinent. Here we report a multicentric 3-year study resulting in the isolation and detailed characterization of 40 strains of Leptospira spp. obtained from infected cattle. Combined serologic and molecular typing identified these isolates as L. interrogans serogroup Pomona serovar Kennewicki (20 strains), L. interrogans serogroup Canicola serovar Canicola (1 strain), L. borgpetersenii serogroup Sejroe serovar Hardjo (10 strains) and L. noguchii (9 strains). The latter showed remarkable phenotypic and genetic variability, belonging to 6 distinct serogroups, including 3 that did not react with a large panel of reference serogrouping antisera. Approximately 20% of cattle sampled in the field were found to be shedding pathogenic Leptospira in their urine, uncovering a threat for public health that is being largely neglected. The two L. interrogans serovars that we isolated from cattle displayed identical genetic signatures to those of human isolates that had previously been obtained from leptospirosis patients. This report of local Leptospira strains shall improve diagnostic tools and the understanding of leptospirosis epidemiology in South America. These strains could also be used as new components within bacterin vaccines to protect against the pathogenic Leptospira strains that are actually circulating, a direct measure to reduce the risk of human leptospirosis.
© 2018 Zarantonelli et al. http://creativecommons.org/licenses/by/4.0/. MenosAbstract:
Leptospirosis is a neglected zoonosis with worldwide distribution. The causative agents are spirochete bacteria of the Leptospira genus, displaying huge diversity of serovars, the identity of which is critical for effective diagnosis and vaccination purposes. Among many other mammalian species, Leptospira infects cattle, eliciting acute signs in calves, and chronic disease in adult animals often leading to abortions. In South America, and including in Uruguay, beef and dairy export are leading sources of national income. Despite the importance of bovine health, food safety, and bovine-related dissemination of leptospirosis to humans, extremely limited information is available as to the identity of Leptospira species and serovars infecting cattle in Uruguay and the South American subcontinent. Here we report a multicentric 3-year study resulting in the isolation and detailed characterization of 40 strains of Leptospira spp. obtained from infected cattle. Combined serologic and molecular typing identified these isolates as L. interrogans serogroup Pomona serovar Kennewicki (20 strains), L. interrogans serogroup Canicola serovar Canicola (1 strain), L. borgpetersenii serogroup Sejroe serovar Hardjo (10 strains) and L. noguchii (9 strains). The latter showed remarkable phenotypic and genetic variability, belonging to 6 distinct serogroups, including 3 that did not react with a large panel of reference serogrouping antisera. Approximately 20% of cattle sampled in the fi... Presentar Todo |
Palabras claves : |
SALUD ANIMAL. |
Thesagro : |
LEPTOSPIRA; LEPTOSPIROSIS. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/11772/1/Zarantonelli-2018-Isolation-of-pathogenic-leptospira-1.pdf
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Marc : |
LEADER 03472naa a2200373 a 4500 001 1059268 005 2018-11-07 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pntd.0006694$2DOI 100 1 $aZARANTONELLI, L. 245 $aIsolation of pathogenic Leptospira strains from naturally infected cattle in Uruguay reveals high serovar diversity, and uncovers a relevant risk for human leptospirosis. 260 $c2018 500 $aArticle History: Received: February 8, 2018; Accepted: July 16, 2018; Published: September 13, 2018. 520 $aAbstract: Leptospirosis is a neglected zoonosis with worldwide distribution. The causative agents are spirochete bacteria of the Leptospira genus, displaying huge diversity of serovars, the identity of which is critical for effective diagnosis and vaccination purposes. Among many other mammalian species, Leptospira infects cattle, eliciting acute signs in calves, and chronic disease in adult animals often leading to abortions. In South America, and including in Uruguay, beef and dairy export are leading sources of national income. Despite the importance of bovine health, food safety, and bovine-related dissemination of leptospirosis to humans, extremely limited information is available as to the identity of Leptospira species and serovars infecting cattle in Uruguay and the South American subcontinent. Here we report a multicentric 3-year study resulting in the isolation and detailed characterization of 40 strains of Leptospira spp. obtained from infected cattle. Combined serologic and molecular typing identified these isolates as L. interrogans serogroup Pomona serovar Kennewicki (20 strains), L. interrogans serogroup Canicola serovar Canicola (1 strain), L. borgpetersenii serogroup Sejroe serovar Hardjo (10 strains) and L. noguchii (9 strains). The latter showed remarkable phenotypic and genetic variability, belonging to 6 distinct serogroups, including 3 that did not react with a large panel of reference serogrouping antisera. Approximately 20% of cattle sampled in the field were found to be shedding pathogenic Leptospira in their urine, uncovering a threat for public health that is being largely neglected. The two L. interrogans serovars that we isolated from cattle displayed identical genetic signatures to those of human isolates that had previously been obtained from leptospirosis patients. This report of local Leptospira strains shall improve diagnostic tools and the understanding of leptospirosis epidemiology in South America. These strains could also be used as new components within bacterin vaccines to protect against the pathogenic Leptospira strains that are actually circulating, a direct measure to reduce the risk of human leptospirosis. © 2018 Zarantonelli et al. http://creativecommons.org/licenses/by/4.0/. 650 $aLEPTOSPIRA 650 $aLEPTOSPIROSIS 653 $aSALUD ANIMAL 700 1 $aSUANES, A. 700 1 $aMENY, P. 700 1 $aBURONI, F. 700 1 $aSALVARREY,X. 700 1 $aBRIANO , C. 700 1 $aASHFIELD, N. 700 1 $aSILVEIRA, C.S. 700 1 $aDUTRA, F. 700 1 $aEASTON, C. 700 1 $aFRAGA, M. 700 1 $aGIANNITTI, F. 700 1 $aHAMOND, C. 700 1 $aMACÍAS-RIOSECO, M. 700 1 $aMENÉNDEZ, C. 700 1 $aMORTOLA, A. 700 1 $aPICARDEAU, M. 773 $tPLoS Neglected Tropical Diseases, September 2018, vol. 12, Issue 9, Article number e0006694. OPEN ACCESS.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
16/09/2014 |
Actualizado : |
18/06/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
A - 1 |
Autor : |
VÁZQUEZ, D.; BERGER, A.G.; CUNIBERTI , M.; BAINOTTI , C.; ZAVARIZ DE MIRANDA , M.; SCHEEREN , P.L.; JOBET, C.; ZÚÑIGA, J.; CABRERA, G.; VERGES, R.; PEÑA, R.J |
Afiliación : |
DANIEL VÁZQUEZ PEYRONEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRES GUSTAVO BERGER RICCA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Influence of cultivar and environment on quality of Latin American wheats. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Journal of cereal science , v. 56, n.2, p. 196-203, 2012. |
DOI : |
10.1016/j.jcs.2012.03.004 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 August 2011 / Received in revised form / 20 March 2012 / Accepted 27 March 2012. |
Contenido : |
ABSTRACT.
Wheat consumption is growing, with processors asking for wheat-based products showing better and more consistent quality. Genotype, environment and their interaction (G #1; E) play an important role in the final expression of quality attributes. An international research consortium was developed in order to evaluate the magnitude of genotype, environment and G #1; E effects on wheat quality of cultivars developed for different agro-ecological zones in Latin America. Genotypes released in Argentina, Brazil, Chile, Mexico, Paraguay and Uruguay, were cultivated in twenty different environments within the participating countries. Each environment was characterized for cultural practices, soil type and climatic conditions. Grain yield and analyses of test weight, protein, ash, gluten, Alveograph, Farinograph, Falling Number, SDS sedimentation and flour color were determined. Allelic variations of puroindolines and
glutenins were determined in all the genotypes evaluated. Both puroindoline and gluten protein alleles corresponded to genotypes possessing medium to very good bread making quality. Large variability for most quality attributes evaluated was observed, with wider ranges in quality parameters across environments than among genotypes; even for parameters known to be largely determined by genotype. The importance of growing environment on grain quality was proved, suggesting that breeders’ quality objectives should be adapted to the targeted environments.
#1; 2012 Elsevier Ltd. All rights reserved MenosABSTRACT.
Wheat consumption is growing, with processors asking for wheat-based products showing better and more consistent quality. Genotype, environment and their interaction (G #1; E) play an important role in the final expression of quality attributes. An international research consortium was developed in order to evaluate the magnitude of genotype, environment and G #1; E effects on wheat quality of cultivars developed for different agro-ecological zones in Latin America. Genotypes released in Argentina, Brazil, Chile, Mexico, Paraguay and Uruguay, were cultivated in twenty different environments within the participating countries. Each environment was characterized for cultural practices, soil type and climatic conditions. Grain yield and analyses of test weight, protein, ash, gluten, Alveograph, Farinograph, Falling Number, SDS sedimentation and flour color were determined. Allelic variations of puroindolines and
glutenins were determined in all the genotypes evaluated. Both puroindoline and gluten protein alleles corresponded to genotypes possessing medium to very good bread making quality. Large variability for most quality attributes evaluated was observed, with wider ranges in quality parameters across environments than among genotypes; even for parameters known to be largely determined by genotype. The importance of growing environment on grain quality was proved, suggesting that breeders’ quality objectives should be adapted to the targeted environments.
#1; 201... Presentar Todo |
Palabras claves : |
CALIDAD DE TRIGO; GENOTIPO; INTERACCIÓN GXE; MEDIO AMBIENTE. |
Thesagro : |
MEJORAMIENTO CULTIVOS DE INVIERNO. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02557naa a2200325 a 4500 001 1050322 005 2019-06-18 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1016/j.jcs.2012.03.004$2DOI 100 1 $aVÁZQUEZ, D. 245 $aInfluence of cultivar and environment on quality of Latin American wheats.$h[electronic resource] 260 $c2012 500 $aArticle history: Received 15 August 2011 / Received in revised form / 20 March 2012 / Accepted 27 March 2012. 520 $aABSTRACT. Wheat consumption is growing, with processors asking for wheat-based products showing better and more consistent quality. Genotype, environment and their interaction (G #1; E) play an important role in the final expression of quality attributes. An international research consortium was developed in order to evaluate the magnitude of genotype, environment and G #1; E effects on wheat quality of cultivars developed for different agro-ecological zones in Latin America. Genotypes released in Argentina, Brazil, Chile, Mexico, Paraguay and Uruguay, were cultivated in twenty different environments within the participating countries. Each environment was characterized for cultural practices, soil type and climatic conditions. Grain yield and analyses of test weight, protein, ash, gluten, Alveograph, Farinograph, Falling Number, SDS sedimentation and flour color were determined. Allelic variations of puroindolines and glutenins were determined in all the genotypes evaluated. Both puroindoline and gluten protein alleles corresponded to genotypes possessing medium to very good bread making quality. Large variability for most quality attributes evaluated was observed, with wider ranges in quality parameters across environments than among genotypes; even for parameters known to be largely determined by genotype. The importance of growing environment on grain quality was proved, suggesting that breeders’ quality objectives should be adapted to the targeted environments. #1; 2012 Elsevier Ltd. All rights reserved 650 $aMEJORAMIENTO CULTIVOS DE INVIERNO 653 $aCALIDAD DE TRIGO 653 $aGENOTIPO 653 $aINTERACCIÓN GXE 653 $aMEDIO AMBIENTE 700 1 $aBERGER, A.G. 700 1 $aCUNIBERTI , M. 700 1 $aBAINOTTI , C. 700 1 $aZAVARIZ DE MIRANDA , M. 700 1 $aSCHEEREN , P.L. 700 1 $aJOBET, C. 700 1 $aZÚÑIGA, J. 700 1 $aCABRERA, G. 700 1 $aVERGES, R. 700 1 $aPEÑA, R.J 773 $tJournal of cereal science$gv. 56, n.2, p. 196-203, 2012.
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